Kumar Sinniah


Education

  • BSc University of Colombo, Sri-Lanka Chemistry (First-class honors), 1984

  • PhD University of Pittsburgh, Physical Chemistry, 1991.

Thesis: Laser induced thermal desorption of molecules from surfaces
Thesis Advisor: Professor Kenneth C. Janda and Professor John T. Yates, Jr.

Professional History

  • Calvin College, 1995-present, Professor of Chemistry
  • University of Michigan, Ann Arbor , 2008-2009, Visiting Professor
  • University of Cambridge, UK, 2001-2002 , Visiting Professor, Department of Chemistry
  • University of Maryland , 1998 Summer, Visiting Scientist, Department of Chemistry
  • University of Maryland and NIST, 1996 Summer, Visiting Scientist, Department of Chemistry
  • University of Maryland at College Park, 1992–1995, Postdoctoral Research Fellow

Biography

Weekly Schedule
(Calvin Portal login required)

Research Groups & Students

Academic interests

The focus of my research group is on single molecule analysis of biomolecular interactions by atomic force microscopy methods. Single molecule analysis provides a unique insight into biological processes that cannot be obtained by conventional methods that provides average measurements from an ensemble of molecules. In our research group, we use single molecule force spectroscopy to study the biophysical interactions between enzymes and inhibitors, and in DNA molecules.

Courses taught

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Research and scholarship

Publications

Undergraduate student names appear in bold.                        

Anna Plantinga, Amanda Witte, Ming-Hsin Li, Andrew Harmon,* Seok Ki Choi, Mark M. Banaszak Holl, Brad G.Orr, James R. Baker, Jr., and Kumar Sinniah, "Bioanalytical screening of riboflavin antagonists for targeted drug delivery - A kinetic and thermodynamic study", ACS Med. Chem. Lett. 2, 2011; 363-367.

Anna Plantinga, Amanda Witte, Seok-Ki Choi, and Kumar Sinniah, "Characterizing riboflavin antagonists for targeted drug delivery", Biophys. J. 100, 2011; S1: 553a.

Christine Timmer and Kumar Sinniah, "Investigating the binding of insulin to G-quadruplex DNA by differential scanning calorimetry", Biophys. J. 100, 2011; S1: 231a.

Kumar Sinniah, Susanna Lynch, Heather Baker, Sarah Byker, Dejian Zhou, "A single molecule study of Guanine Quadruplex DNA" Biophys. J. 98, 2010; S1: 592a.

Susanna Lynch, Rachel Battershell, Jordan Scott, Dejian Zhou, and Kumar Sinniah, "A single molecule force spectroscopy study of the binding interaction between insulin and G-quadruplex DNA" Biophys. J. 98, 2010; S1: 592a.

L. Devon Triplett, Daniel Q. McNerny, J. Gabriel Grodzicki, Kumar Sinniah, Mark M. Banaszak Holl, Bradford G. Orr, "Investigating the interaction between folic acid and folate binding protein at the single molecule level", Biophys. J. 98, 2010; S1: 596a.

Susanna Lynch, Heather Baker, Sarah Byker, Dejian Zhou, and Kumar Sinniah, "Single molecule force spectroscopy on G-quadruplex DNA," Chem. Euro. J. 15, 2009; 8113-8116.

Susanna Lynch, Heather Baker, Sarah Byker, Dejian Zhou, and Kumar Sinniah, "Single Molecule Force Spectroscopy of Guanine Quadruplex DNA," Biophys. J. 96, 2009, 36a.

Kumar Sinniah, Laura Porter-Peden, Sarah Byker, Ronald Blankespoor, Dejian Zhou, Chris Abell, and Trevor Rayment, "A Single Molecule Study of Enzyme-Inhibitor Interactions," Biophys. J. 96, 2009;643a.

Laura Porter-Peden, Sarah Kamper, Mark Vander Wal, Ronald Blankespoor, and Kumar Sinniah, "Estimating kinetic and thermodynamic parameters from single molecule enzyme inhibitor interactions", Langmuir 24, 2008; 11556-11561.

Jennifer Nichols, Joey Buthker, and Kumar Sinniah, "The Effect of Divalent Cations (Mg2+, Zn2+, Mn2+, and Ca2+) on the Hybridization Efficiency Of DNA,"  Biophys. J. 94, 2008; 1712.

Sarah Kamper, Laura Porter-Peden, Ronald Blankespoor, Mark Vander Wal, and Kumar Sinniah, "The Effect of Tether Length in Single Molecule Force Spectroscopy," Biophys. J. 94, 2008; 1714.

Laura Porter-Peden, Sarah Kamper, Kumar Sinniah, Ronald Blankespoor, Dejian Zhou, Chris Abell, and Trevor Rayment, "Kinetics and Thermodynamics of Enzyme-Inhibitor Interactions," Biophys. J. 94, 2008; 1720.

Sarah Kamper, Laura Porter-Peden, Ronald Blankespoor, Kumar Sinniah, Dejian Zhou, Chris Abell, and Trevor Rayment, "Investigating the specific interactions between carbonic anhydrase and a sulfonamide inhibitor by single molecule force spectroscopy", Langmuir 23, 2007; 12561-12565.

Xiuzhu Wang, Dejian Zhou, Kumar Sinniah, Caroline Clarke, Louis Birch, H. Li, Trevor Rayment, Chris Abell, "Electrostatic orientation of enzymes on surfaces for ligand screening probed by force spectroscopy", Langmuir 22, 2006; 887-892.

Mark Vander Wal, Sarah Kamper, Jennifer Headley, and Kumar Sinniah, "Effect of contact force and salt concentration on the unbinding of a DNA duplex by force spectroscopy", Langmuir 22, 2006; 882-886 .

Dejian Zhou, Kumar Sinniah, Chris Abell, and Trevor Rayment, "Label-free detection of DNA hybridization at the nanoscale: An AFM based highly sensitive and selective method", Angewandte Chemie International Edition 42, 2003; 4934-4937.

Kimberly Aumann, Karen C. J. Muyskens, and Kumar Sinniah, "Visualizing Atoms, Molecules, and Surfaces by Scanning Probe Microscopy", J. Chem. Educ. 80, 2003; 187.

Dejian Zhou, Kumar Sinniah, Chris Abell and Trevor Rayment, "The Use of Atomic Force Microscopy for Making Addresses in DNA Coatings", Langmuir 18, 2002; 8278.

Jamie Paauw, John Ubels, and Kumar Sinniah, "Investigating Live Fixed Chang Conjunctival and Corneal Fibroblast Cells by Atomic Force Microscopy", Current Eye Research 25, 2002; 61.

Mark Schotanus, Kimberly Aumann, and Kumar Sinniah, "Using Force Spectroscopy to Investigate the Binding of Complementary DNA in the Presence of Intercalating Agents", Langmuir 18, 2002; 5333-5336.

Kumar Sinniah and Kenneth Piers, "Ion Chromatography: Analysis of Ions in Pond Waters", J. Chem. Educ. 78, 2001; 358.

Steve Robey and Kumar Sinniah, "Initial etching of GaAs (001) during hydrogen plasma etching" Journal of Applied Physics 88, 2000; 2994.

Presentations (since 2001)

Laura Porter-Peden, Sarah Kamper, Kumar Sinniah, Ronald Blankespoor, Dejian Zhou, Chris Abell, and Trevor Rayment, "Kinetics and thermodynamics of enzyme-inhibitor interactions", Joint Meeting of the Biophysical Society 52 nd Annual Meeting & 16 th International Biophysics Congress, Long Beach, CA, February 2-6, 2008. (Laura Porter-Peden was a Biophysical Society Travel Award Winner)

Sarah Kamper, Laura Porter-Peden, Ronald Blankespoor, Stephanie Hoogendorn, Mark Vander Wal, and Kumar Sinniah, "The effect of tether length in single molecule force spectroscopy", Joint Meeting of the Biophysical Society 52 nd Annual Meeting & 16 th International Biophysics Congress, Long Beach, CA, February 2-6, 2008. (Sarah Kamper was a Biophysical Society Travel Award Winner)

Jennifer Nichols, Joseph Buthker, and Kumar Sinniah, "The effect of divalent cations (Mg2+ , Zn2+ , Mn2+ , and Ca2+ ) on the hybridization efficiency of DNA", Joint Meeting of the Biophysical Society 52 nd Annual Meeting & 16 th International Biophysics Congress, Long Beach, CA, February 2-6, 2008.

Sarah Kamper, Laura Porter-Peden, Kumar Sinniah, Ronald Blankespoor, Chris Abell, and Trevor Rayment, "A single molecule study of enzyme-inhibitor interactions", 233rd ACS National Meeting, Chicago, March 25-29, 2007.

Joseph Buthker, Jennifer Nichols, and Kumar Sinniah, "Determining the effects of divalent cations on DNA using AFM", 233rd ACS National Meeting, Chicago, March 25-29, 2007.

Mark Vander Wal, Sarah G. Kamper, Jennifer Headley, and Kumar Sinniah, "Factors affecting the study of biomolecular interactions", 37 th Great Lakes Regional Meeting of the American Chemical Society, Milwaukee, WI, May 31-June 2, 2006.

Sarah G. Kamper, Jennifer Headley, Mark Vander Wal, and Kumar Sinniah, "Is force spectroscopy an effective method for discriminating the binding of drugs to DNA?", 37 th Great Lakes Regional Meeting of the American Chemical Society, Milwaukee, WI, May 31-June 2, 2006.

Kumar Sinniah, Sarah Kamper, Laura Porter-Peden, Xiuzhu Wang, Dejian Zhou, Chris Abell, and Trevor Rayment, "Investigating enzyme inhibitor interactions by force spectroscopy", CUR 2006 National Conference, DePauw University, Indiana, June 24-27, 2006.

Sabina Thyle, Mark Schotanus, Kimberly Aumann, and Kumar Sinniah, "Investigation of drug-DNA interactions by Force Spectroscopy", The Michigan Academy of Science, Arts, and Letters 2004 Meeting, March 5, 2004.

Sabina Thyle, Mark Vander Wal, Tamar Hunse, Mark Schotanus, Kimberly Aumann, and Kumar Sinniah, "Investigating drug-DNA interactions by force spectroscopy", Council on Undergraduate Research, 10 th National Conference, June 23-26, 2004.

Eric Arnoys, Carissa Hockema, and Kumar Sinniah, "Visualization of F-Actin by Scanning Probe Microscopy", 33 rd Great Lakes ACS Central Regional Meeting, June 11-13, 2001.

Kumar Sinniah, Joel Visser, and Karen Gilliland, "Analysis of volatile organics from a painting studio", 33 rd Great Lakes ACS Central Regional Meeting, June 11-13, 2001.

Julia Heetderks, Kumar Sinniah, Melvin Schindler, and Marcos Dantus, "Investigations of Streptavidin-Biotin binding by Atomic Force Microscopy", 33rd Great Lakes ACS Central Regional Meeting, June 11-13, 2001.

Kumar Sinniah, Jamie Paauw, and John Ubels, "Use of Atomic Force Microscopy to observe cells in culture", 33rd Great Lakes ACS Central Regional Meeting, June 11-13, 2001.

Kimberly Aumann, Kumar Sinniah, and Elizabeth Howell, "DNA binding of replication factor C and proliferating cell nuclear antigen as imaged using atomic force microscopy", 33rd Great Lakes ACS Central Regional Meeting, June 11-13, 2001.

Current Students

 


Rachel Battershell
(2009-2010)
Chemistry and French Major


Anna Plantinga
(2009-2010)
Biology and Math Major


Stephenie Graf
(2010)
Chemistry Major


Christie Timmer
(2010)
Biology Major


Amanda Witte
(2010)
Chemistry & Biochemistry Major

Past Students

Matt Salie (2010 Spring)
Biochemistry Major

 


Susanna Lynch
(2008-2009)
NIH IRTA Fellowship


Jordan Scott
(2009)

PhD Student in Biochemistry
Notre Dame University


Heather Baker
(2008)
Senior Biochemistry
Calvin College


Sarah (Kamper) Byker
(2006-2008)
PhD Student in Chemistry
Northwestern University

Laura Bolton (2007-2008)
PhD Student in Chemistry
University of Washington-Seattle


Mark Vander Wal
(2005-2007)
PhD Student in Chemistry
Princeton University


Jennifer Nichols
(2005-2007)
PhD Student in Toxicology
University of North Carolina-Chapel Hill


Joseph Buthker
(2006-2007)
PhD Student in Chemistry
University of Illinois-Urbana Champaign


Dena Vander Tuig
(2006-2007)
MD/MPH Program
Emory University, Atlanta


Gladys Kamanga-Sollo
(2007)
MD Program
George Washington University, DC


Emily Tubergen
(2007)
Teach for America


Rachel Veltman (2006)


Tamar Hunse (2004-2006)
MD Program
Saba University, Netherlands-Antilles


Helen Etya'ale (2006)
MPH - 2008 Global Epidemiology
Emory University, Atlanta, GA


Ryan Colburn (2005-2006)
DVM Program - Small and Exotic Animal Medicine
Michigan State University


Sabina Thyle
(2004)
MD Program
University of Southern California


Lisa (Green) Rasbach (2003)
MSN, Pediatric Nurse, South Carolina

Lisa Boyle (2002)
Acupuncturist
Los Angeles, CA

Mark Schotanus (2001-2002)
Bio Lab Technician
Calvin College


Kimberly Arnold (2000-2001)
MS, Chemistry Instructor
Indiana University, Bloomington


Julia Heetderks, PhD (2000-2001)


Joel Visser (1998-2001)
JD/MS in Environmental Policy and Planning, University of Michigan, Ann Arbor


Jamie Paauw (2000-2001)
Physician, Ophthalmology
Texas A&M University


Carissa Hockema Cheng (1999-2000)
Registered Dietician


Karen Gilliland (1999)


Stacy (Vander Kooi) Bajema (1999)
Technology Consultant
Innovative Technology Solutions, MN

Sam Glass, PhD (1997)

Tom Vos, PhD (1997)
Invista, Orange, TX

Eric Achtyes, MD (1997)
Psychiatry
Pine Rest Christian Mental Health Service, Grand Rapids, MI

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