Proteins and the Nuclei of Mammalian Cells
Our research goals is to determine how specific proteins shuttle in and out of the nucleus of mammalian cells.
We have been using molecular biology, fluorescence microscopy with Green Fluorescent Protein (GFP), and molecular modeling to examine how the polarity of amino acids and the structure of proteins allow them to bind to interact with transport proteins.
Confocal fluorescence microscopy also allows us to selectively bleach GFP in specific regions or compartments within the cell. The rate of return of fluorescence depends upon the protein being studied, the compartment bleached, and the state of the cell. We hope to use this information to help determine how cells regulate protein transport within the cell.
- Course code: